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KMID : 0811720070110000073
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Korean Journal of Physiology & Pharmacology
2007 Volume.11 No. 0 p.73 ~ p.0
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Role of Amino Acid Transporters LAT1 and LAT2 during Odontoblast Differentiation
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Kim Do-Kyung
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Abstract
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Dentin is a mineralized tissue formed by odontoblasts that are differentiated from ectomesenchymal cells. The molecular mechanism of differentiation in odontoblasts remains unclear. Amino acid transporters play an important role in supplying nutrition to cells including odntoblasts. Amino acid transport system L is a major nutrient transport system responsible for the transport of neutral amino acids including several essential amino acids. The system L is divided into two major subgroups, the L-type amino acid transporter 1 (LAT1) and the L-type amino acid transporter 2 (LAT2). In this study, the expression pattern and role of amino acid transport system L were, therefore, investigated in the differentiation of MDPC-23 cells derived from mouse dental papilla cells. The LAT1 mRNA was expressed in the early stage of MDPC-23 cell differentiation. The expression level was gradually increased by time course and it was decreased after the late stage. The LAT2 mRNA was not observed in the early stage of MDPC-23 cell differentiation. The LAT2 mRNA was expressed at the 11 days of MDPC-23 cell differentiation and the expression level was gradually decreased by time course. The expression of ON mRNA was gradually decreased but the expression of ALP mRNA was increased during differentiation of MDPC-23 cells. The L-leucine uptake was increased by time course from the early stage to the 9 days in MDPC-23 cell differentiation. The amount of L-leucine uptake was maintained to the 11 and 14 days of MDPC-23 cell differentiation. As the results, it is considered that among neutral amino acid transport system L in differentiation of MDPC-23 cells, the LAT1 has a key role in cell proliferation in the early stage and middle stage of cell differentiation and the LAT2 has an important role in cell differentiation and mineralization in the late stage of cell differentiation for providing cells with neutral amino acids including several essential amino acids.
Source: Korean Journal of Physiology & Pharmacology.2007 Oct;11(Suppl II):S75-S75
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KEYWORD
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Amino acid transport system L, Odontoblast, Proliferation, Differentiation, Mineralization
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